Senior Research Assistant | Vector Development and Construction
Wellcome Trust Sanger Institute
Ref: 81071
Salary range £27,589 to £38,067 per annum dependent on experience.
The Wellcome Trust Sanger Institute is a world leader in genomic research, with an expanding scientific programme dedicated to understanding gene function in health & disease.
With the sequencing of the mouse and human genomes, the next major challenge is the functional annotation of mammalian genes to elucidate their detailed roles in both normal biology and disease.
The Sanger Mouse Genetics Program (MGP) is establishing a new pipeline for engineering custom mutations in mice by gene targeting in embryonic stem(ES) cells. This highly focused pipeline will apply cutting edge gene targeting technology to create mutant alleles of interest to Sanger Institute Faculty in their hypothesis driven research projects, many of which involve the modeling of human disease. Our efforts will benefit from the extensive targeting vector/ES cell modular resources generated as part of the KOMP, EUCOMM, and EUCOMMTOOLs high throughput gene targeting programs and associated expertise.
We are seeking a highly motivated and experienced individual to join the team. The post holder’s main responsibilities will be to manage and execute the day-to-day workflows of projects for generation of targeting vectors and quality assured targeted ES cells for MGP custom Faculty gene targeting projects. This includes the design, execution and delivery of these resources as well as database records maintenance and and projects troubleshooting. A commitment to both consistent quality and technical innovation would be expected in this role.
Essential Skills
- Science degree in biological sciences
- Significant proven experience in murine ES cell culture
- Practical knowledge of genotyping techniques (PCR, LOA, Southern blots)
- Experience and understanding in developing gene targeting strategies and executing them from start to finish
- An understanding of genome browsers and basic bioinformatics
- Experience in molecular biology techniques, preferably including recombineering
- Ability to schedule and deliver projects within agreed deadlines
- Good communication skills to allow accurate information to be exchanged with others
- Up to date knowledge of the field of gene targeting including relevant literature
- Ability to test and implement new methodologies including troubleshooting
Ideal Skills
Prior proven ability to manage a group of laboratory technicians would be advantageous
Benefits
The Institute has excellent purpose built facilities on the Genome Campus, Hinxton on the outskirts of Cambridge. We offer a comprehensive range of benefits including a final salary pension scheme and excellent on-site facilities. Further details can be found on our website https://jobs.sanger.ac.uk.
To submit your CV and apply for this job please go to
https://jobs.sanger.ac.uk, to register and apply on line.
Closing date: 22 February 2012.
Senior Research Assistant | Vector Development and Construction
Wellcome Trust Sanger Institute
Ref: 81071
Salary range £27,589 to £38,067 per annum dependent on experience.
The Wellcome Trust Sanger Institute is a world leader in genomic research, with an expanding scientific programme dedicated to understanding gene function in health & disease.
With the sequencing of the mouse and human genomes, the next major challenge is the functional annotation of mammalian genes to elucidate their detailed roles in both normal biology and disease.
The Sanger Mouse Genetics Program (MGP) is establishing a new pipeline for engineering custom mutations in mice by gene targeting in embryonic stem(ES) cells. This highly focused pipeline will apply cutting edge gene targeting technology to create mutant alleles of interest to Sanger Institute Faculty in their hypothesis driven research projects, many of which involve the modeling of human disease. Our efforts will benefit from the extensive targeting vector/ES cell modular resources generated as part of the KOMP, EUCOMM, and EUCOMMTOOLs high throughput gene targeting programs and associated expertise.
We are seeking a highly motivated and experienced individual to join the team. The post holder’s main responsibilities will be to manage and execute the day-to-day workflows of projects for generation of targeting vectors and quality assured targeted ES cells for MGP custom Faculty gene targeting projects. This includes the design, execution and delivery of these resources as well as database records maintenance and and projects troubleshooting. A commitment to both consistent quality and technical innovation would be expected in this role.
Essential Skills
- Science degree in biological sciences
- Significant proven experience in murine ES cell culture
- Practical knowledge of genotyping techniques (PCR, LOA, Southern blots)
- Experience and understanding in developing gene targeting strategies and executing them from start to finish
- An understanding of genome browsers and basic bioinformatics
- Experience in molecular biology techniques, preferably including recombineering
- Ability to schedule and deliver projects within agreed deadlines
- Good communication skills to allow accurate information to be exchanged with others
- Up to date knowledge of the field of gene targeting including relevant literature
- Ability to test and implement new methodologies including troubleshooting
Ideal Skills
Prior proven ability to manage a group of laboratory technicians would be advantageous
Benefits
The Institute has excellent purpose built facilities on the Genome Campus, Hinxton on the outskirts of Cambridge. We offer a comprehensive range of benefits including a final salary pension scheme and excellent on-site facilities. Further details can be found on our website https://jobs.sanger.ac.uk.
Closing date: 22 February 2012.